The study on SDF-1α and c-MYC protein regulate tachykinin receptor 1-trucated mRNA expression
10.3760/cma.j.issn.0254-5101.2011.03.011
- VernacularTitle:SDF-1α和c-MYC蛋白调控速激肽受体-1截短型mRNA表达的研究
- Author:
Tie XIONG
;
Yunli ZHOU
;
Zhi YAO
- Publication Type:Journal Article
- Keywords:
Stromal cell derived factor 1α;
Tachykinin receptor 1;
shRNA;
c-myc gene
- From:
Chinese Journal of Microbiology and Immunology
2011;(3):241-244
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate how SDF-1α and c-MYC protein regulates TACRl-Tr expression. Methods c-myc shRNA vector was constructed, small interfering RNA was employed for silencing c-myc gene in MCF-7 breast cancer cell. SDF-1α neutralized antibody was used in c-myc+ cell group and c-myc- cell group, while other c-myc+ cell group and c-myc- cells group were cultured under normal condition. The mRNA level of TACRl-Tr was determined by real-time PCR. Results c-myc shRNA vector was constructed successfully, in the normal presence of SDF-la, the level of TACRl-Tr mRNA in c-myc- cell group were lower than that in c-myc+ cell group( P < 0.05). But in the presence of SDF-la neutralized antibody, TACRl-Tr mRNA level of c-myc- cell group was higher than that of c-myc+ cell group(P < 0.05). Conclusion In the normal culture condition, c-MYC protein may transactivate TACRl-Tr transcription in MCF-7 cell, in the presence of SDF-1α neutral antibody, c-MYC protein lost the activity of transactivating for TACRl-Tr transciption.
