In vitro study on Coxsackie virus B3 infection on islet cells

VernacularTitle:柯萨奇病毒B组3型感染胰岛细胞的体外研究
Author: Mingwei SHENG ; Sisi CHEN ; Sheng BAI ; Yu ZHENG ; Xiaomian LI
Publication Type:Journal Article
Keywords: Coxsackie virus; Bone marrow; Mesenchymal stem cells; Islet cells; Diabetes
From: Chinese Journal of Microbiology and Immunology 2011;(3):229-233
CountryChina
Language:Chinese
Abstract: Objective To study the effect of Coxsackie virus B3 (CVB3) infection on islet cells in vitro, and to explore the mechanism of islet cells caused by CVB3. Methods Bone marrow mesenchymal stem cells( BMSCs) were separated from the bone marrow and cultured. Then they were induced to differentiate into islet-like cells using nicotinamide and mercaptoethanol. Differentiated cells were detected by morphology , special staining and RT-PCR. Observe CVB3 infection on islet cells under inverse microscope and detect the specific gene fragment by RT-PCR. Results BMSCs showed half suspended shape and gathered to form a cluster after induction. Cells became red brown by dithizone specific staining. RT-PCR also proved the existence of mRNA expressing insulin. Infected islet cells appeared typical pathological changes like shrinks, refraction decreases. RT-PCR detected the desired specific gene fragment of 299 bp in infected islet cells. Conclusion CVB3 can directly injury islet cells, and damage the function of islet cells of secreting insulin.