Anti-tumor effects of p53N15-based fusion peptide in the transfected H1299 lung cancer cells
10.3760/cma.j.issn.1001-4497.2011.04.016
- VernacularTitle:p53N15融合肽转染肺腺癌细胞系H1299的抗肿瘤作用
- Author:
Hongtao WANG
;
Xucang DU
;
Shudong LI
;
Yawei DOU
;
Yanfei CAO
;
Wei TIAN
;
Quanying WANG
;
Guangxiao YANG
- Publication Type:Journal Article
- Keywords:
Lung neoplasms;
Gene therapy;
Genes,p53;
Fusion peptide
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2011;27(4):232-235
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveLoss-of-function mutation of p53,a tumor suppressor gene,is an important mechanism for the development of human cancers.In this study we tried to transfect p53N15-based fusion peptide into H1299,a lung cancer cell line,and evaluate the anti-tumor effects of the fusion peptide.MethodsAdeno-associated virus ( AAV) vectors were used for transfecting p53N15 fusion peptide into p53-null lung adenocarcinoma H1299 cells.The anti-replication effects of p53N15 fusion peptide were evaluated with inverted microscopy,MTT test for cell viability and flow cytometry.ResultsFusion peptides in H1299 cells was highly expressed and had detectable suppressive effects on cell proliferation.A large amount of dead cells were seen under microscope after the transfection of recombinant viruses for 72 hours.Cells activity was reduced significantly in the virus-transfecting groups as demonstrated by MTT test.The flow cytometry showed that a large number of dead cells were present in the virus-transfecting groups.ConclusionThe growth of H1299 lung adenocarcinoma cells could be inhibited in vitro by being transfected with p53N15 fusion peptide,which may be a potential gene therapy alone or as an adjuvant option in the treatment of lung cancer.
