DETERMINATION OF CHOLESTEROL IN HUMAN SERUM BY COULOMETRIC TITRATION
- VernacularTitle:库仑滴定法测定血清胆固醇的含量
- Author:
Zhiming WEN
;
Airu LIU
;
Lixin XU
- Publication Type:Journal Article
- Keywords:
cholesterol;
serum;
coulometric titration
- From:
Acta Pharmaceutica Sinica
2000;35(4):297-300
- CountryChina
- Language:Chinese
-
Abstract:
To study the determination method of cholesterol in human serum by Coulometric titration. METHODS: The titration was carried out in an electrolytic solution containing 1 mol.L-1 potassium bromide and glacial acetic acid (1∶3). Bromine was electrogenerated at the anode and cholesterol reacted with bromine with a value of reactional electrons (n) as 2. RESULTS: The ranges of recovery for 0.115 mg to 0.459 mg of cholesterol were from 93.9% to 105%, and the average recovery was 100.3%. The relative standard deviation in all applied analysis was less than 1.5%. The accuracy of the method was also assessed in comparison with the results obtained by the enzymatic method used clinically. CONCLUSION: The method proposed is simple, rapid and accurate, and is suitable for the determination of cholesterol in human serum samples.
