Density and Affinity of IL-6 Receptors in Human Leukemic Cells
- VernacularTitle:IL-6受体在人白血病细胞膜上的表达及亲和力
- Author:
Shuang LIU
;
Yongzhi XI
;
Siqi GUO
;
Nan LIU
;
Min TU
;
Li JIN
;
Xingguo CHEN
;
Fanhua KON
- Publication Type:Journal Article
- Keywords:
leukemic cells;
interleukin-6 receptor;
radial ligand binding assay;
affinity
- From:
Chinese Journal of Cancer Biotherapy
2000;7(4):255-260
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To make a study of density and affinity of IL-6R in human leukemic cell lines, and discuss the affection of high affinity IL-6R to the targeted treatment of leukemia with IL-6-PE40 fusion protein. Methods: Radial binding assay with scatchard plot and FACS were used to analysis the density and affinity of IL-6R and protein expression of IL-6Rα and β subunits in totally 8 representative human leukemic cell lines. Results: Myelocytie, monocytic and erythrocytic leukemic cell lines U937, HL-60, KG1 and TF1 express high affinity IL-6R, whose average density per cell is 2 502,2 874, 2 319 and 9 329 respectively, however no 125I-IL-6 binding was detected on chronic myelocytic leukemic cell line K562 and lymphoblastic leukemic cell lines such as Raji, CEM and HUT28. These results correlate with those of FACS highly. Conclusion:These observations suggest that acute nonlymphoblastic leukemic cells may be more suitable for targeted treatment with IL-6-PFA0 fusion protein.
