Construction of Mutant N-ras/61 Gene Recombinant Vaccinia Virus
- VernacularTitle:突变N-ras基因重组痘苗病毒rV-N-ras/61的构建
- Author:
Jian ZHANG
;
Yueran ZHAO
;
Li YOU
;
Chunyi GAO
;
Zhigang TIAN
- Publication Type:Journal Article
- Keywords:
vaccinia virus;
N-ras gene;
mutant;
vaccine
- From:
Chinese Journal of Cancer Biotherapy
2000;7(4):282-284
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct the recombinant vaccinia virus of mutant N-ras/61 gene and enhance the immunogenecity of mutant N-ras／61 protein produced by the recombinant vaccinia virus. Methods: N-ras／61 gene was inserted into P1108 and transfected into CV-1 cell infected with vaccinia virus by Cell FECTIN. PCR and Western blot were used to identify the recombinants. Results: We get recombinant vaccinia virus rV-N-ras/61 by PCR and tk- selecting. The expression of N-ras gene was detected by Western blot. Conclusion: This study is a test for studing effective vaccine of mutant N-ras/61 gene. The efficacy in vivo of the N-ras／61 vaccine in immunotherapy is under investgation.
