Cloning, Expression and Activity of Recombinant Human Angiostatin
- VernacularTitle:重组人血管抑素的克隆、表达及活性测定
- Author:
Ying JIANG
;
Luguo SUN
;
Yongli YU
- Publication Type:Journal Article
- Keywords:
angiostatin;
expression
- From:
Chinese Journal of Cancer Biotherapy
2000;7(4):285-287
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To express recombinant human angiostatin for further application in clinic. Methods: The complete encoding eDNA of human angiostatin was isolated from human embryo liver with RT-PCR and expressed in secretory Pichia expression system. Recombinant human angiostatin was purified with heparin sepharose chromatography and its activity was determined in chick embryo chorioallantoic membrane (CAM) and wound healing assays. Results: Expressed in large quantity (yield=5 mg/L) and purified with heparin sepharose, recombinant angiostatin was showed to have a molecular weight of 43 kD in SDS-PAGE and potently inhibit angiogenesis and wound healing. Conclusion: Recombinant human angiostatin was expressed efficiently in a biologically active form.
