EFFECT OF bFGF ON THE PROLIFERATION AND DIFFERENTIATION OF THE NEURAL PROGENITOR OF EMBRYONIC HIPPOCAMPUS OF RATS IN VITRO
- VernacularTitle:碱性成纤维细胞生长因子对胚鼠海列神经前体细胞的增殖和分化作用
- Author:
Yiwen RUAN
;
Chuanen WANG
;
Yao XIE
;
Ningli WANG
;
Zhibin YAO
- Publication Type:Journal Article
- From:
Chinese Journal of Neuroanatomy
2000;16(3):201-204
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate effect of basic fibroblast growth factor(bFGF) on the prolifiration and differentiation of theneural progenitor of embryonic hippocampus of rats in vitro, 25 ng/ml bFGF was employed into the serum-free medium of cultureof hippocampal neural cells of embryonic day 18 rats in the present study. The effect of bFGF on the viability of cells culturedwas detected by MTT colorimetric method and the effects of bFGF on proliferation and differentiation of hippocampal neural pro-genitors were analyzed qualitatively and quantitatively by means of immunochemistry, for the nestin, neurofilament, galactocere-broside and glial acidic fibrillary protein. The results showed that the OD value of experimental group was higher than that ofcontrol group by 1.5 and 1.8 times at 4 d and 8 d respectively. The quantitative analysis of each kinds of cells indicated that thenumber of neural progenitors, neurons and oligodendrocytes in experimental group were increased about 2 times as many as thatin control group, but no differences of astrocytes between the two groups at 4 d. However, the number of four kinds of cells aug-mented about 1.7 times at 8 d. The results of this study suggest that bFGF can not only promote the survival, proliferation, butalso facilitate the differentiation of neural progenitor of hippocampus to neurons and glial cells. To obtain more many purifiedneural progenitors in vitro, the embryonic day 18 is not an appropriate age. It is better to get younger embryo brain to culture and to add enough bFGF.
