CONSTRUCTION AND IDENTIFICATION OF RECOMBINANT RETROVIRAL VECTOR CARRYING RAT GDNF GENE
- VernacularTitle:GDNF基因逆转录病毒表达载体的构建与鉴定
- Author:
Chuanen WANG
;
Yiwen RUAN
;
Zhibin YAO
;
Yao XIE
;
Huiyu GUO
- Publication Type:Journal Article
- Keywords:
GDNF;
retroviral vectors;
DNA recombination;
gene therapy;
rat
- From:
Chinese Journal of Neuroanatomy
2000;16(3):209-212
- CountryChina
- Language:Chinese
-
Abstract:
Through genetic recombination technique, the rat glial cell line-derived neurotrophic factor (rGDNF) cDNA was in-serted into polylinker site of retroviral vector pLXSN, to generate a recombinant plasmid pLXSN-GDNF as transfer vector. Therecombinant plasmid was verified with restriction analysis, PCR, dot blot hybridization and Southern blot hybridization. The re-sults showed that GDNF cDNA was cloned correctly into retroviral vector pLXSN, recombinant retroviral vector was construct-ed. It is concluded that the eukaryotic cell expression vector was constructed successfully for gene therapy of Parkinson's,Alzheimer's and other central nervous system diseases.
