THE TRICHROME STAIN AND IMMUNOCYTOCHEMICAL REACTION TO DEMONSTRATE NEURITE AND SCHWANN'S CELL IN CULTURED NERVE TISSUE
- VernacularTitle:三色染色法和免疫细胞化学法显示培养的神经轴索及雪旺细胞
- Author:
Xiaodong WANG
;
Xiaosong GU
;
Peiyun ZHANG
;
Dong WANG
;
Guangming LU
- Publication Type:Journal Article
- Keywords:
S-100 protein;
nerurofilament;
cell culture;
trichrome stain;
immunocytochemistry;
nerve tissue;
rat
- From:
Chinese Journal of Neuroanatomy
2000;16(3):231-233
- CountryChina
- Language:Chinese
-
Abstract:
The special trichrome stain and immunocytochemical stain were used to show neurites, Schwann's cells in cultured pe-ripheral nerve tissue. The dorsal root ganglia(DRG) of rat were cultured on polypyrrole membrane for 2 weeks. Then, the cul-tured speciments were stained by special stain, which was composed of hematoxylin, fast green FCF. ehromotrope 2R and phos-photungstic acid; or by immunocytochemical stain with anti-S-100 protein and anti-neurofilament antibodies. In the specialtrichrome stained specimen the long processes from DRG were stained aquamarine blue; part of the cell nuclei on the processes orpolypyrrole membrane were stained red or purplish red, and the cytoplasm ashen. We testified that the long processes from DRGwere neurites and the cells which were purplish red nuclei and ashen cytoplasm were Schwann's cells in immunocytochemicalstain. The special staining could differentiate neurites and Schwann's cells in cultured peripheral nerve tissue.
