Analysis of Antibodig Spectrum of Anti- membranous Lahyrinth Proteins in Experimental Animals with Autoimmune Inner- ear Disease
- VernacularTitle:自身免疫性内耳病动物模型的抗内耳特异性抗体谱系分析
- Author:
Pengju WANG
;
Zhanyuan WU
;
Baikui XIAO
- Publication Type:Journal Article
- Keywords:
Autoimmune diseaseAutoantibodyImmunoassayCochlea
- From:
Journal of Audiology and Speech Pathology
2000;8(3):131-133
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo define the components of membranous labyrinth proteins which take part in the development of autoimmunity in experimental animals with autoimmune inner- ear disease(AIED). MethodsAn animal modee of AIED was es tablished by using homologous crude inner ear antigen to immune the guinea pig. The autoantibodies against inner ear antigenin the sera of animals were examined by immunoblotting assay. ResultsThe components of membranous labyrinth proteins were very complicated, which had five main bands in electrophoresis; most of immunized animals had only developed two sorts of autoantibod ies which were anti-68 kD and 136 kD membranous labyrinth proteins. There was no positive reaction in sera of control animals. Conclusion This results showed that the immune reactions against the 68 kD and 136 kD proteins of membranous labyrinth may be autoimmune bases for AIED in animals.
