Effect of Gentamicin on the Expression of Subunit Isoforms of Na, K-ATPase in Stria Vascualris of Guinea Pig
- VernacularTitle:庆大霉素对豚鼠血管纹Na，K-ATP酶α亚单位异构体表达的影响
- Author:
Shixun ZHONG
;
Zhaohua LIU
- Publication Type:Journal Article
- Keywords:
GentamicinNa;
K-exchanging ATPaseStria vascularis
- From:
Journal of Audiology and Speech Pathology
2000;8(3):137-139
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the expression of asubunit isforms of Na,K-ATPase in stria vascularis of guinea pig, and the effect of gentamicin on it. MethodsThe expression of a subunit isoforms of Na, K-ATPase in stria vascualris of guinea pig and its change after gentamicin exposure were identified immunocytochemically using the specific mouse antibodies a gainst the α1 ,α2 and α3 subunits of rat Na,K-ATPase. ResultsAll marginal cells,intermediate cells and basal cells of stria vas cualris showed positive expression of α1 isoform and negative expression of α2 and α3 isoforms. The immunostaining of α1 isform was attenuated significantly after gentamicin esposture. ConclusionGentamicin significantly inhibits the expression of Na, K-ATPase α1 subunit isoform of the stria vascularis, sequentially inhibits the activity of Na,K-ATPase.
