Damage of high glucose on retinal capillary pericytes
- VernacularTitle:高浓度葡萄糖对视网膜毛细血管周细胞的影响
- Author:
Lixia CHENG
;
Yanhu DONG
;
Jiafu WANG
- Publication Type:Journal Article
- Keywords:
retin;
capillary pericytes;
glucose;
cell proliferation
- From:
Recent Advances in Ophthalmology
2000;20(5):339-341
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of high glucose on retinal capillary pericytes in vitro and research the pathogenesis of diabetic retinopathy (DR). Methods Retinas dissected from eyes of fresh killed cattle were separated and retinal capillary pericytes were cultured. The effect of high glucose on pericytes was evaluated by measurement of lactate dehydrogenase(LDH),3H-thymidine incorporation and liquid scintillation techniques.Morphological changes were observed under microscope and electric microscope.Results There was no significant difference in LDH activity in the supernatants between high glucose(10、20、40mmol*L-1) and normal glucose(5mmol*L-1)cultured for 48h or 96h. High glucose had an effective inhibition on pericytes' proliferation cultured for 48h,96h and 144h, and the inhibition rate increased when the glucose concentration rised and time passed by. The inhibition rate of pericytes was 78.09% after cultured in high glucose (40mmol*L-1) for 144h. Conclusion High glucose has an effect inhibition on pericytes' proliferation, and the inhibition rate increase when the glucose concentration rises and time passes by.
