Binding Properties of M-CAT Element in the Chicken Nicotinic Acetylcholine Receptor γ-Subunit Promoter
- VernacularTitle:鸡烟碱样乙酰胆碱受体γ亚基启动子中的M-CAT结合特性
- Author:
Juhua NI
;
Peng LIU
;
Hongti JIA
- Publication Type:Journal Article
- Keywords:
M-CAT element;
M-CAT binding factor;
Acetylcholine receptor γ-subunit promoter
- From:
Chinese Journal of Biochemistry and Molecular Biology
2000;16(5):580-585
- CountryChina
- Language:Chinese
-
Abstract:
Several M-CAT elements are present in the chicken nicotinic acetylcholine receptor γ-subunit promoter and required for the totipotency of this gene.To understand the binding properties of M-CAT element,the interaction of M-CAT motif,at the position -67/-61 relative to the initiation site of γ-promoter with nuclear proteins,were analyzed.Mutageneses and binding experiments demonstrated that the M-CAT core motif bound nuclear protein (s) from tested chicken tissues to generate a high-mobility complex;while the flanking sequence bound different factors to generate lower-mobility complexes with the exception of liver.Southwestern blotting showed that only a 30 kD protein was expressed in tested tissues of chicken embryo at day 13.These results suggest that a 30 kD factor can directly bind to M-CAT element in the manner of monomer,even homodimer and is ubiquitously expressed in tissues,while the binding of the factor(s) to flanking sequence should probably rely on the presence of a 30 kD ubiquitous factor.
