Selecting EGF-binding Clones From a pⅧ-based Phage Display Library
- VernacularTitle:从噬菌体文库中筛选与表皮生长因子结合的多肽
- Author:
Weigang TANG
;
Jiada LI
;
Changlin LI
;
Keyi WANG
;
Renbao GAN
- Publication Type:Journal Article
- Keywords:
phage display;
peptide;
EGF;
drug target
- From:
Progress in Biochemistry and Biophysics
2000;27(4):432-434
- CountryChina
- Language:Chinese
-
Abstract:
Angiogenesis-related diseases involving EGF include acherosclerotic plaques, haemangioma, angiofibroma, tumor growth and arthritis. EGF may serve as a drug target and its antagonists may have important clinical applications.Peptide phage display libraries have been successfully applied in areas of finding ligands for enzymes, receptors, and many other molecules. A pⅧ-based peptide phage display library was panned with the cytokine EGF and several EGF-binding clones were selected based on ELISA and micropanning assays. The selected EGF-binders from peptide phage display library may be utilized in affinity chromatography in EGF downstream processing and even act as potential antagonists of EGF if their affinity is further improved through secondary library strategy.
