Human embryonic neural retina transplantation to rabbit retina
- VernacularTitle:人胚胎视网膜神经上皮移植于兔视网膜的初步实验研究
- Author:
Chunyang ZHAO
- Publication Type:Journal Article
- Keywords:
retina transplantation;
human embryonic neural retina;
rabbit
- From:
Recent Advances in Ophthalmology
2000;20(5):319-321
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the results of the experimental transplantation of human embryonic neural retina to rabbits.Methods Neural retina from human embryo of 2.5～3.5 months were transplanted to the site where choroids and retina were excised through posterior sclera in 6 adult rabbits. 20g*L-1 Cyclosporine oil and 1g*L-1 Dexamethasone eyedrops were locally used to control the rejection 3 times a day postoperatively. The transplants were observed under electron-microscope 3 months later.Results In 5 survival grafts out of 6 cases, the cone and rod cells,a few ganglion cells and relatively a lot of amacrine cells and Muller cells were found under electron-microscope. But the photoreceptors and ganglion cells were immature in development,the synaptic connection was not evident, the inner limiting membrane and nerve fiber layer were not found. The immune cells involved in rejection were not observed.Conclusions The survival rate of the transplants of the human embryonic neural retinas is relatively high, the differentiation of the grafts is confirmed under electron-microscope, but the development of the neural cells is not good enough to maturity. The rejection is controlled in a good state with Cyclosporine and Dexamethasone locally administrated.
