Distribution of sodium pump α-subunit isoforms in normal sprague-dawley rats
- VernacularTitle:正常SD大鼠组织钠泵α亚单位的基因表达
- Author:
Weiqing YUAN
;
Hao WANG
;
Zhuoren LI
- Publication Type:Journal Article
- Keywords:
rats;
sodium pump;
gene
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
2000;21(6):524-527
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the distribution of sodium pump α-subunit isoforms at both mRNA and protein level in normal Sprague-Dawley(SD) rats. MethodsWith RT-PCR (reverse transcription-polymerase chain reaction) and immunohistochemical assay, sodium pump α1-, α2-, andα3-subunit isoforms were detected at mRNA level and protein level in the myocardium, liver, kidney, adrenal gland, aortic smooth muscle, pituitary and hypothalarnus in normal rats. ResultsSodium pump α1-, α2-, and α3-subunit isoforms distribute in a tissue-specific fashion in normal SD rats. α1 and α2 are the main isoforms and α3 is much less in myocardium. α1 is more than α2 or α3 isoform at mRNA level in liver, while all of α1-,α2-, and α3-isoform are less expressed at protein level. α1-isoform is abundantly but α2 and α3 are less expressed both at mRNA and protein levels in kidney. α2isoform is just more than α1 and α3 isoforms in aortic smooth muscle. All of three isoforms are expressed abundantly in pituitary. α2 and α3 are more and α1 is less expressed in hypothalamus. ConclusionThe results of this study have revealed the distribution of sodium pump α-subunit isoforms at both mRNA and protein level in normal SD rats, which might be helpful for the further studying the physiologic and pathologic roles of sodium pump.
