Anti-tumor effect of Ganoderma lucidum karst and its mechanism
- VernacularTitle:灵芝的抗肿瘤作用机制
- Author:
Zhibin LIN
- Publication Type:Journal Article
- Keywords:
Ganoderma lucidum;
polysaccharides;
anti-tumor
- From:
Basic & Clinical Medicine
2000;20(5):391-393
- CountryChina
- Language:Chinese
-
Abstract:
Ganoderma lucidum(Leyss．Ex fr．)Karst．(Lingzhi) is a medicinal fungus with a long history in China as a valuable tonic remedy．Modern Pharmacological and clinical investigation demonstrated that Lingzhi had anti-tumor activities．Recently the antitumor effects of extract of Ganoderma lucidum(GLE) and Ganoderma polysaccharides B(GL-B) and its mechanism were investigated．The results demonstrated that GLE and GL-B significantly inhibited growth of implanted sarcoma 180 in vivo．GL-B also promoted anti-tumor activity induced by cyclophosphamide in mice in vivo．GLE and GL-B directly adding to tumor cells-cultured medium neither suppressed sarcoma 180 and HL-60 proliferation nor induced apoptosis of both tumor cells in vitro．However the serum from GLE and GL-B treated mice can suppress S-180 and HL-60 cells proliferation and induced its apoptosis in vitro．Furthermore splenocytes conditioned medium with GL-B (GL-B-S-CM) and peritoneal macrophages conditional medium with GL-B (GL-B-PM-CM) also significantly inhibited HL-60 proliferation and induced its apoptosis in vitro．Further study indicated that GLE and GL-B could promote TNFa production from murine peritoneal macrophages and IFNg production from murine spleen cells in vitro．Finally GLE and GL-B could promote TNFa mRNA expression in murine peritoneal macrophages and IFNg mRNA expression in murine spleen cells．The results suggest that the anti-tumor effect of Ganoderma lucidum relates to activating macrophage and spleen cell，then promoting TNFa mRNA expression and IFNg mRNA expression，finally resulting TNFa and IFNg release．
