Study on the methods of qualitative diagnosis of laryngeal displasia and early carcinoma
- VernacularTitle:喉粘膜上皮不典型增生定性诊断和早期喉癌诊断方法的探讨
- Author:
Yinping WANG
;
Yudan HAO
;
Xiaofeng GUO
;
Baodong DU
- Publication Type:Journal Article
- Keywords:
displasia;
carcinoma;
p53 gene;
collagen Ⅳ;
AgNORs
- From:
Journal of Jilin University(Medicine Edition)
2000;26(6):621-623
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the methods of early qualitative diagnosis of laryngeal displasia and earlycarcinoma. Methods:We investigated displasia (34 cases),squamous cell carcimoa (19 cases) and vocalcord polyps (17 cases) of larynx which were embeded in paraffin by PCR－SSCP analysis of p53 gene,im－munochemi stain of p53 protein and Ⅳ collagen,cytometer (determining fraction of cell cycle) and AgNORstain. Results :Eleven of 34 cases of displasia were all positive in the indexes. Seven of 11 cases were alsopositive in pathology,but others were negative. Five of 34 cases of displasia were all negative in the index-es,2 of 5 cases were positive and 1 of 5 cases were negative,other two cases did not have following-up re-sults in pathology. Conclusion :The results suggest that it is the combination of the morphological indexeswith biological indexe that is more scientific and more accurate in quatitative diagnosis of premalignantchange.
