Effects of Drugs on Ovarian Granulosa Cell in Rats
- VernacularTitle:药物对大鼠卵巢颗粒细胞的影响
- Author:
Meijuan WEI
;
Jin WANG
;
Jin YU
- Publication Type:Journal Article
- Keywords:
female rats;
granulosa cell culture;
sex hormone measurement
- From:
Fudan University Journal of Medical Sciences
2000;27(6):514-516
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the effects of antibiotics and herbs with removing pathogenic heat from blood on ovarian granulosa cell morphological and functions in female rats. Methods The granulosa cell of 30 d age SD female rats was collected,several drugs were added to the culture fluid.The culture fluid were collected to measure the sex hormone by radioimmunoassay.The cell organs of granulosa cell were measured by transmission electron microscope. Results Organs of the granulosa cell almost disappeared and perinuclear vesicle were founded in Amikacin sulfate group and Rhizoma zedoariae group.The progesterone levels were lower than in salline group (P<0.05).The estrogen levels of Amikacin group were lower than in saline group (P<0.05). Conclusions Ovarian function were obstructed in female rats when Amikacin,Rhizoma zedoariae were directly used.
