Quantitative Determination of Saponins in the Root of Panax pseudo-ginseng var.notoginseng by HPLC-ELSD and UV Spectrophotometry
- VernacularTitle:HPLC-ELSD及紫外分光光度法测定三七中皂苷的含量
- Author:
Yingqiao JIANG
;
Qiang WANG
;
Shiping MA
;
Xuedong DANG
- Publication Type:Journal Article
- Keywords:
Panax pseudo-ginseng var. notoginseng (Burkill) Hoo et Tseng HPLC-ELSD gin-senoside Rg1 total saponins UV spectrophotometry
- From:
Chinese Traditional and Herbal Drugs
2000;31(10):737-739
- CountryChina
- Language:Chinese
-
Abstract:
A reverse phase HPLC-ELSD method for the determination of ginsenoside Rg1 in the rootof Panaa pseudo-ginseng var. notoginseng (Burkill) Hoo et Tseng was reported. Chromatographic condi-tions: Shim-pack CLC-ODS column (6.0 mm×150 mm)； acetonitrile-water (30: 70) as the mobilephase； Shimadzu LC-6A with SEDEX-55 ELSD detector. The method was found to be simple and accuratewith recovery rate of 100. 50％ and RSD= 1.82 %. The established UV spectrophotometric determinationof total saponins in P. pseudo-ginseng var. notoginseng was also tried and gave an accurate result coinci-dental with that of the HPLC results. The recovery rate was 101.50%， and RSD=1. 44%. It seemed thatboth methods can be used reliably for the quality control of P. pseudo-ginseng var. notoginseng.
