Influence of Blood Replenishing Angelica Decoction on Enhanced Endothelial Permeability Induced by Histamine
- VernacularTitle:当归补血汤对组胺引起的血管内皮细胞单层通透性增高的作用
- Author:
Zhibi HU
;
Yi FAN
;
Zhifen HAN
;
Dazheng WU
;
Chunqing SONG
- Publication Type:Journal Article
- Keywords:
Danggui Buxue Tang (DGBXT)(Blood Replenishing Angelica Decoction) histamine endothelial monolayer permeability
- From:
Chinese Traditional and Herbal Drugs
2000;31(10):756-758
- CountryChina
- Language:Chinese
-
Abstract:
Blood Replenishing Angelica Decoction [Danggui Buxue Tang (DGBXT)]is a wellknown TCM prescription composed of Radix Angelicae Sinensis and Radix Astragali with the actions ofinvigorating "Qi" and enriching blood. Its action to curtail endothelial permeability induced by histaminewas studied. Endothelial cells isolated from the aorta of neonatal calf were cultured on polycarbonate mi-croporus filter membrane to develop a confluent endothelial monolayer. After purfused with either plainHank's balanced salt solution or that containing 5 g/L albumin, the monolayer was treated with 10-4 mol/L histamine for 30 min either with or without preincubation for 60 min with 10-4 g/mL of DGBXT. Fluidfiltration coefficient (Kf), filtration volume (Jv) and osmotic reflective coefficient (σ) of protein were thenmeasured. The findings showed that DGBXT could curtail the lowering of Kf and Jv and elevation of σ in-duced by histamine, indicating that DGBXT could inhibit the action of histamine on endothelial permeabili-ty, but its mechanism of action needs further study.
