Metallothionein involvement in myocardial protection of basic fibroblast growth factor
- VernacularTitle:金属硫蛋白参与碱性成纤维细胞生长因子的心肌细胞保护作用
- Author:
Shulian LI
;
Xiaohong WANG
;
Mingui FU
;
Yongzheng PANG
;
Chaoshu TANG
- Publication Type:Journal Article
- Keywords:
Metallothionein;
Fibroblast growth factor,basic;
Anoxia;
Reperfusion
- From:
Chinese Journal of Pathophysiology
2000;16(12):1260-1262
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe whether metallothionein plays a role in cardiac protective effect of basic fibroblast growth factor on anoxic/reperfusion (A/R) injury in cultured cardiomyocytes and study the possible mechanism of cardiac protection by bFGF.METHODS: The present study made the model of myocyte A/R injury after having a 24 h incubation by bFGF( 10-10、10-9、10-s mol/L) and bFGF( 10-9 mol/L) + PD098059 respectively. We measured the levels of MT and MDA in myocytes, and the changes of LDH and protein in cultured medium. We also counted the number of viable cell in groups. RESULTS: The contents of myocardial MT were significantly increased after treatment by bFGF. The levels of MT in I0-l0 mol/L、10-9 mol/L and 10-8 mol/L bFGF treated groups increased 54 %、 62%、 76% respectively, compared with the A/R group, and the number of viable cell were also greatly increased, LDH and protein leakage in cultured medium and MDA contents in myocyte were dramatically decreased in bFGF treated groups. All the protection were completely disappeared with the inhibition of MT production with PD098059, theinhibitor of mitogen- activated protein kinase(MAPK). CONCLUSION: MT involves in the protection of bFGF in cultured cardiomyocytes. It might be related with activation of MAPKase.
