Inhibition of ICAM- 1 expression on endothelial cells inhypoxia/reoxygenation by antisense oligodeoxynucleotides
- VernacularTitle:反义寡核苷酸抑制缺氧／再给氧时内皮细胞细胞间粘附分子-1的表达
- Author:
Bin XIA
;
Hongshi XU
;
Fuyuan SHAO
;
Lei CHEN
- Publication Type:Journal Article
- Keywords:
Oligonucleotides,antisense;
Anoxia;
Endothelium,vascular;
Flow cytome.try
- From:
Chinese Journal of Pathophysiology
2000;16(12):1267-1269
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of antisense oligodeoxynucleotides (AS - ODN) on the intercellsular adhesion molecule- 1 (ICAM- 1 ) expression on endothelial cells in hypoxia/reoxygenation(H/R). METHODS: With cultured glomerular vascular endothelial cell in H/R, the positive percentage of ICAM - 1 expression was measured by flow cytometry before and after giving AS - ODN. RESULTS: The ICAM - 1 expression did not increase on glomerular vascular endothelial cell in 10 hours hypoxia compared to control group, it increased in 6 hours reoxygenation, and decreased by 40.6 % after giving AS- OND. CONCLUSION: AS - ODN may decrease the expression of ICAM- 1 on endothelial cells in H/R.
