Effect of ATP on proliferation and membrane protein expression in immortalized hmnan fibroblasts
- VernacularTitle:ATP对人不死化成纤维细胞增殖及其细胞膜蛋白表达的影响
- Author:
Junwu LI
- Publication Type:Journal Article
- Keywords:
Adenosine triphosphate;
Cells;
Membrane proteins
- From:
Chinese Journal of Pathophysiology
2000;16(12):1270-1271
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of ATP on proliferation and membrane protein expression in immortalized human fibroblasts. METHODS: Applying the normal human fibroblast cell line TIG - 7 and OUMS - 36, the immortalized human fibmblast cell line KMST - 6 and SUSM - 1 to culture for 24 hours and 96 hours with the different concentrations of ATP, ADP and AMP, respectively. The number of cell alive, DNA synthesis and the expression of [32p] - ATP labeled proteins in the membrane fraction were also observed. RESULTS: After 96 hours culture, the proliferation inhibition in immortalized cells exposed to 0.4mmol/L ATP was 77 %, while that of normal OUMS36 cells treated with the same concentration of ATP was 41%; the DNA synthesis of the immortalized cells exposed to 0.4mmol/L ATP was greater suppressed than that of normal OUMS - 36 cells. ADP was the only reagent tested that reduced the number of cells significantly( P < 0.01 ). When the immortalized cells exposed to ADP, AMP, adenosine, and phosphoric acid. In the presence of 1 mmol/L ATP, many KMST - 6 cells died, but normal OUMS - 36 cells did not. Compared with normal cells, the expresson of [32p] - ATP labeled 30 kD, 31 kD, 33 kD and 40 kD proteins were markedly higher in the immortalized cells. CONCLUSION: ATP inhibited the proliferation and increased membrane protein expression in immortalized human fibroblasts.
