The clinical application of fiuorescently - labeled monoclonal antibody against P- selectin
- VernacularTitle:抗P一选择素单抗的荧光素标记及其临床应用
- Author:
Yiming ZHAO
;
Mingqing ZHU
;
Yang HE
;
Wenhong SHENG
;
Changgeng RUAN
- Publication Type:Journal Article
- Keywords:
Antibodies,monoclonal;
Fluoresceins;
Flow cytometry;
Cell adhesion molecules
- From:
Chinese Journal of Pathophysiology
2000;16(12):1289-1291
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the clinical significance in determination of the P- selectin levels in subjects with prethrombotic state or thrombosis by flow cytometry (FCM). METHODS: The P- selectin expression on platelet membrane in 42 patients with diabetes mellitus, 33 with hyperlipidemia, 23 with cerebral infarction and 20 healthy individuals, were analyzed using fluorescently - labeled SZ - 51 by direct FCM comparing with indirect FCM and enzyme- linked immunosorbent assay (ELISA). RESULTS: The level of P- selectin on platelet membrane is higher in DM (23.92 % + 15.83 % ), in hyperlipidemia ( 18.34 % + 9.46 % ) and in cerebral infarction ( 19.32 % + 10.38 % ) than normal subjects (3.38 % + 1.11% ) ( P < 0.01 ). In addition, similar results on P - selectin were obtained by indirect FCM and ELISA in patients with DM and cerebral infarction. CONCLUSION: FITC - labeled SZ - 51 - IgG can be used in FCM, and it would be a new and sensitive method in detecting platelet activation.
