Effects of nitric oxide on mitochondrial damage caused by exogenous calcium
- VernacularTitle:NO在外源性高浓度Ca2+损伤心肌线粒体中的作用
- Author:
Wanyi LIANG
;
Zongcheng YANG
;
Yuesheng HUANG
- Publication Type:Journal Article
- Keywords:
Mitochondria;
Calcium;
Nitric oxide
- From:
Chinese Journal of Pathophysiology
2000;16(12):1292-1294
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of nitric oxide (NO) on mitochondrial damage caused by exogenous calcium. METHODS: Normal myocardial mitochondria were divided into three groups; L- arginine control group (CG), Ca2 + - damaged group (DG) and L - NAME - preserved group (PG). Mitochondria of all groups were incubated at 30℃ with reaction medium containing 20μmol/L EDTA, 100μmol/L CaC12 and 1 μmol/L L- NAME with 100μmol/L CaCl2 respectively. Then the NO2-/NO3- contents, mitochondrial viability and membrane potential were investigated. RESULTS: The NO2-/NO3 contents of DG was obviously higher than that of CG and PG, meanwhile, there was no obvious difference between CG and PG. Mitochondrial viability and membrane potential of DG were significantly lower than that of CG and PG, and negatively related to NO2-/NO3- contents ( r = - 0.5297, P < 0.01; r = -0.6041, P < 0.01 ). But, the mitochondrial viability and membrane potential of PG were still lower than that of CG. CONCLUSION: Exogenous calcium could activate mitochondrial nitric oxide synthase resulting in NO production and the latter play an important role in decreasing mitochondrial viability and membrane potential.
