Sequence Analysis of SBR Gene in Expressional Plasmid of Transgenic Plant
- VernacularTitle:转基因植物表达质粒中SBR基因的序列分析
- Author:
Shaoyun JIANG
;
Junqi LING
- Publication Type:Journal Article
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2001;22(1):48-50
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To sequence P1 gene(184～1946 bp) includ ing SBR gene in expressional plasmid of transgenic plant. 【Methods】 pROP1 plas mid was extracted from E. coli DH5α, P1 gene was sequenced by PCR sequence kit and DNA autosequencer and compared with surface protein gene of Streptococc us mutans in GenBank. 【Results】 616 base pairs from 5′ end and 466 base pairs from 3′ end were sequenced, its accuracy is up to 98%. 【Conclusion】 The seq uence of P1 gene's 5′ end and 3′ end is consistent with DNA sequence of SBR re gion of Streptococcus mutans surface protein pac gene and may provide useful in formations to construct the transgenic plant anticaries vaccine.
