Polymorphic Analysis of DYS390 and DYS389Ⅰ/Ⅱ in Han Nationality in Liaoning Province in China
- VernacularTitle:中国辽宁汉族DYS390、DYS389Ⅰ/Ⅱ基因座及单倍型研究
- Author:
Yan LU
;
Baojie WANG
;
Mei DING
;
Hao PANG
;
Dacheng SUN
- Publication Type:Journal Article
- From:
Journal of China Medical University
2001;30(1):25-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Our aim was to investigate the polymorphism and forensic meaning of the Y-chromosomal loci DYS390 and DYS389Ⅰ/Ⅱ in Han nationality in Liaoning Province. Methods: By amplifying these loci with polymorase chain reaction (PCR) followed by polyacrylamide gel electrophoresis and silver staining, the allele frequencies of these loci among 98 unrelated men of Han nationality were investigated. Results: Five alleles with different frequencies from 0.010 to 0.480 were detected of DYS390， and three alleles with different frequencies from 0.224 to 0.439 were detected of DYS 389Ⅰ. Locus DYS 389Ⅱ was detected in five alleles and the frequencies ranged from 0.020 to 0.367. The probability discrimination of these loci were 0.637, 0.644 and 0.723. These loci were found to generate at least 31 different haplotypes and the power of discrimination was 0.948. Conclusion: The loci DYS390 and DYS389Ⅰ/Ⅱ are very important when detect mixed samples, especially in deficiency cases of paternity test.
