The inhibitory effects on the growth of human hepatocellular carcinoma cell and its mechanism by theasinesin
- VernacularTitle:聚酯型儿茶素对人肝癌细胞生长的抑制作用及其机制
- Author:
Huawen XIN
;
Fandian ZENG
;
Bijun XIE
- Publication Type:Journal Article
- From:
Chinese Pharmacological Bulletin
2001;17(1):37-40
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the inhibitory effects on the growth of SMMC-7721 human hepatocellular carcinoma cell line and its mechanism by theasinesin. METHODS MTT assay,colony formation test, 3H-TdR, 3H-Leu incorporation test, determination of the intracellular cAMP and cGMP level, in situ hybridization were used. RESULTS Theasinesin at 50 mg*L-1 could significantly inhibit the growth and colony formation of SMMC-7721 cells. Theasinesin could strongly inhibit DNA and protein synthesis and increase intracellular cAMP levels. C-myc gene expression was significantly decreased and p53 gene increased strongly in a dose-dependent manner.CONCLUSION Theasinesin demonstrates significant inhibitory effects on the growth of SMMC-7721 cells. Its mechanisms may involve the inhibition of DNA and protein synthesis ,the elevation of intracellular cAMP levels, inhibition of c-myc gene expression and enhancement of p53 gene expression.
