Effect of quateranary ammonium salt derivative (F2) of haloperidol on calcium in vascular smooth muscle cells
- VernacularTitle:氟哌啶醇季铵盐衍生物对血管平滑肌细胞钙离子的影响
- Author:
Risheng WEI
;
Ganggang SHI
;
Yingna ZHANG
- Publication Type:Journal Article
- From:
Chinese Pharmacological Bulletin
2001;17(1):54-56
- CountryChina
- Language:Chinese
-
Abstract:
AIM To observe the effect of quateranary ammonium salt derivative (F2) of haloperidol on calcium level in vascular smooth muscle cells (VSMCs) isolated from thoracic aortas of rat. METHODS VSMCs were loaded with Fluo-3-AM, a calcium sensitive fluorescent dye, and ［Ca2+］i was determined by the use of laser scanning confocal microscope (LCSM). RESULTS In Ca2+ (1.26 mmol*L-1) bath solution, intracellular calcium fluorescent intensity (FI) in VSMCs was increased when application with 30 mmol*L-1 KCl, and then was inhibited after addition with F2. The FI of the ASMCs with different concentrations of F2 (0.1,1,10,100 μmmol*L-1)within three minutes were 64%±9%(n=16, P<0.05),16%±5%(n=20, P<0.01),0.6%±0.8%(n=20, P<0.01),0.1%±0.3%(n=15, P<0.01), respectively. CONCLUSION F2 could decrease the intracellular Ca2+ level in the VSMCs by blocking the voltage-dependent calcium channel.
