Nitric Oxide Inhibits the Synthesis of Type Ⅱ Collagen in Rabbit Cultured Chondrocytes
- VernacularTitle:一氧化氮抑制兔关节软骨细胞II型胶原的合成
- Author:
Xinxin SHAO
;
Dexiang TIAN
;
Changlong YU
;
Qiming CHEN
- Publication Type:Journal Article
- From:
Chinese Journal of Sports Medicine
2001;20(1):31-34
- CountryChina
- Language:Chinese
-
Abstract:
Nitric oxide (NO) plays an important pathophysiological role in osteoarthritis and cartilage metabolism. To determine the relationship between NO and the synthesis of type II collagen in cartilage, we measured levels of type II collagen by ELISA and procollagen (II) mRNA by RT-PCR in cultured lapine chondrocytes that were incubated with some kinds of reagents. 0.2mM sodium nitroprusside (SNP, a NO donor) can release high levels of NO, decreasing type II collagen, suppressing the expression of procollagen (II) mRNA (COL2A1).At the same time, chondrocytes showed a large increase in NO synthesis, a decrease in type II collagen and COL2A1 mRNA in response to 100u/ml IL-1. When 1mg/ml N-nitro-L-arginine methyl easter (NAME, an inhibitor of NO synthase) was mixed with IL-1, NO production was inhibited, the amounts of type II collagen recovered partially and COL2A1 mRNA recovered completely.These data indicate NO can inhibit type II collagen synthesis as IL-1 downstream molecule by suppressing procollagen (II) mRNA.
