Preparation and molecular weight estimation of ouabain pol yclonal antibody F(ab)2 fragment
- VernacularTitle:哇巴因多克隆抗体F(ab)2片段的制备及其分子量测定
- Author:
Mingjuan ZHANG
;
Zhuoren LU
;
Yukang YUAN
;
Langchong HE
;
Hao WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(1):5-7
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim is to prepare ouabain polyclonal antibody F(ab)2 fragment and to estimate its molecular weight.Methods[ KG*2 [ WTBZ]Ouabain polyclonal antibody was obtained from immunized rabbits.The antibod y was digested with pepsin.The resulting products were analyzed and the molecular weig ht of F(ab)2 fragment was estimated by HPSEC.The immune activity was detec ted by ELISA.Results 100 mg of ouabain polyclonal antibody wa s dige sted by 2 mg of pepsin for 18 hours at pH 3.0 and active ouabain polyclonal anti body F(ab)2 framgment was obtained.Its molecular weight was 107 kD.Concl usion The active ouabain polyclonal antibody F(ab)2 fragment coul d be prepared by digesting its antibody with pepsin.
