Study on determination method of the rhG-CSF biological activity
- VernacularTitle:重组人粒细胞集落刺激因子生物学活性测定方法研究
- Author:
Qian JIA
;
Xingjun ZHOU
;
Xuehong TIAN
;
Yuling FAN
;
Xiaobo QI
;
Zhihua ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(1):25-26
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The determination method of rhG-CSF bio logical activity by NFS-60 cells was studied in accordance with WHO internation al standard for G-CSF.Methods MTT method was adopted.R esults The chomosone number of NFS-60 cell was 39, about 1×105 cells/ml to 1×107 cells/ml,the dying color showed gradient when the NFS-60 c ells was dyed by MTT,the method(4,4) was adopted in the determination of rhG-CS F biological activity,the average FL% of potency was 13.560% for single exprimen t,and the CV of inter-assay and intra-assay was lower than 10% and 10.109%,respectively.Conclusion method(4,4) can be us ed in the determination of rhG-CSF biological activity,and the results can guid e the study and the manufacture of rhG-CSF effectively.
