Optimization of liquid culture conditions of dihydropyrimidinase producing strain Pseudomonas putida 9801
- VernacularTitle:二氢嘧啶酶产生菌培养条件的研究
- Author:
Honghui HUANG
;
Jun LI
;
Zhuoyi HU
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(1):15-17
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim is to optimize the liquid culture conditions of dihydropyrimidinase producing strain Pseudomonas putida 9801. Methods Plackett-Burman design and spherical symmetric desig n were used.Results Optimum conditions for dihydropyrimidinas e formation of Pseudomonas putida 9801 were defined:yeast extract 2.39%, Glu cose 1.81%,Uracil 0.06%,K2HPO4*3H2O 0.2%, MgCl2*6H2O 0.05%and NaCl 0 .3%,when the strain was cultured at 32℃ for 10 h,about 3.02 units/ml of hydanto inase was obtained. This value was quite consistent with the theory value(2.91 u nits/ml).Conclusion The liquid culture conditions of dihydrop yrimidinase producing strain were optimized.
