Purification of antifungal pepitde from Aspergillus fumigatus
- VernacularTitle:烟曲霉菌中抗真菌活性肽类物质的分离与纯化
- Author:
Zhezhu JIN
;
Yushu WANG
;
Jingling JIN
;
Yingji CAI
;
Hekui JIN
;
Zhengyong JIN
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(1):13-14
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim is to purify the peptide with antifu ngal activity from Aspergillus fumigatus culture fluid.Methods The pept ide was separated by ion exchange column chromatography and further purified by r everse phase HPLC,and the molecular weight was determined by tricine gel electro phoresis.Results A kind of peptide(FIP) with antifungal activit y wa s separated. The molecular weight was about 8 000. The detection result by MTT method showed significant antifungal activity of the peptide to 5 kinds of fung i.Conclusion The study provided a reliable basis for developin g drug and antiseptic.
