A new method for the determination of the activity of HGF
- VernacularTitle:一种促肝细胞生长因子活性测定方法
- Author:
Yang JI
;
Runjie ZHANG
;
Yanan WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(1):10-12
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim is to develop a new method for the determination of the activity of hepatocyte growth factor (HGF). Met hods Rat hepatocytes were prep ared by collagenase perfusion and incubated at 5% CO2 incubator for 1.5 h. The n HGF was added into the culture medium and incubated at 5% CO2 incubator for 1.5 h. The cell viability was determined by MTT method.Results ～1 142 μg/ml improved the number of cell viability significantly （AHGF/Acontrol） ＞2.Conclusion Th is new method was stable, time-saving and of good reappearance. It was adapted to the determination of HGF activity.
