Protective effect of nerve growth factor (NGF) from the Chinese cobra venom on spinal neurons after sciatic nerve lesion
- VernacularTitle:中华眼镜蛇毒神经生长因子对神经元的保护作用
- Author:
Minjian CHEN
;
Bin YANG
;
Chunyan ZHANG
;
Yuyan SHU
- Publication Type:Journal Article
- Keywords:
nerve growth factor;
sciatic nerve;
image analysis
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2001;6(1):5-7
- CountryChina
- Language:Chinese
-
Abstract:
AimTo investigate the protective effect of nerve growth factor (NGF) from the Chinese cobra venom on spinal neurons after sciatic nerve lesion. Methods Fourty Wistar rats were divided into 2 groups each containing 20 animals. After surgical crush lesion on the bilateral sciatic nerve, the rats were injected NGF into the target muscle every day. On the d 5 and d 10 after operation, enzyme histochemistry technique was used to show acetycholinesterase(AchE) activity of the motoneurons in L5～S2 spinal cord anterior hornandfluorideresistantacidphophatase (FRAP) activity of substantia gelatinosa in L5～ S2 spinal cord posterior horn. The activity of enzyme was analysed with computer image analysis system. Results Compared with the control group, the activity of FRAP in NGF group was higher on the d 5 and d 10 after operation and the activity of AchE in NGF group was higher on the d 10 after operation. ConclusionIt is demonstrated that NGF could protect the mononeurons in spinal cord anterior horn and the sensory neurons in dorsal root ganglia after axotomy.
