Effects of the total extract of astragalosides on the growth, AFP secretion and γ-GT activity of human hepatoma cells
- VernacularTitle:黄芪总提物对肝癌细胞生长、甲胎蛋白分泌及γ-GT活性的影响
- Author:
Yan YANG
;
Shaogang SONG
;
Minzhu CHEN
- Publication Type:Journal Article
- Keywords:
hepatoma cells;
AFP;
γ-GT activity;
astragalosides
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2001;6(1):18-20
- CountryChina
- Language:Chinese
-
Abstract:
AimTo study the effect of the total extract of astragalosides(TEA) on the growth, AFP secretion and γ-GT activity of human hepatoma cells invitro.Methods The human hepatoma HepG2 and Bel-7404 cells were cultured with TEA 5～160 mg · L-1 for 6 days. Anti-hepatoma activity was measured by MTT method. AFP was assaysed by ELISA and γ-GT was determined by enzyme-substrate method. Results The growth of the human hepatoma HepG2 and Bel-7404 cells and the secretion of AFP of HepG2 cells were reduced significantly by TEA 5～160 mg· L-1. The γ-GT activity of HepG2 cells was inhibited and its ALB content was increased by TEA 20, 40 and 80 mg · L-1. The γ-GT activity of Bel-7404 cell was inhibited and its ALB content was increased byTEA 40 and 80 mg · L-1 Conclusion These results suggest that TEA has inhibitory effects on the growth, AFP secretion and γ-GT activity of human hepatoma cells.
