Anti-inflammatory effect of astragalosides and its mechanisms of action
- VernacularTitle:黄芪总甙的抗炎作用及其作用机制探索
- Author:
Qin YANG
;
Jingtao LU
;
Bin WANG
;
Aiwu ZHOU
;
Minzhu CHEN
- Publication Type:Journal Article
- Keywords:
astragalosides;
carrageenan;
an ti-inflammation;
interlukin 8;
phospholipases A2
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2001;6(1):21-24
- CountryChina
- Language:Chinese
-
Abstract:
AimTo study the anti-inflammatory effect of astragalosides(AST) and its mechanisms of action. Methods The exudate volume, neutrophil count and protein content in exudate were measuredinthe carrageenan-induced air pounch model in rats. The content of PGE2 was assayed by radioimmunoassay,the activity of PLA2 by microacid titration assay, IL-8 by ELISA, and NO by nitrate reductase assay. The production of O2 in neutrophil was determined by cytochrome C assay. Results AST(40, 80 mg· kg -1) could markedly reduce the exudate volume, neutrophil count, protein content, the content of IL-8, and the production of O2.AST lowered PLA2 activity of neutrophil and accellular component in exudate, and it also decreased the contents of PGE2 and NO in exudate. Conclusion AST has an obvious anti-inflammatory effect on carrageenan-induced acute inflammation.Its mechanisms may be related to the inhibition of vascular permeability and leukocyte migration, as well as to the suppression of PLA2 activity and the reduction of IL-8, PGE2, NO and O2 production.
