Generation of recombinant adenovirus vector containing human p16(Ad-p16)and titer detection of the adenovirus
- VernacularTitle:重组腺病毒Ad-p16的扩增及病毒滴度检测
- Author:
Wenquan ZHOU
;
Zhilian MIN
;
Li LI
;
Lingzhen ZHANG
- Publication Type:Journal Article
- From:
Journal of Medical Postgraduates
2001;14(1):44-46
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To investigate the method of generating recombinant adenovirus vector containing human p16(Ad-p16) efficiently. Methods:Recombinant adenovirus vector was generated with HEK 293 cells, and the titer of the adenovirus was detected. Results:The titer of generated adenovirus is about 5×108 PFU/ml. Conclusions:Recombinant adenovirus vector was generated with HEK 293 cells with high titer. This set the foundation of adenovirus mediated gene-transfer.
