INHIBITORY EFFECT OF BOANMYCIN ON THE GROWTH OF COLON CARCINOMA 26 AND HEPATIC METASTASIS IN MICE
- VernacularTitle:博安霉素对小鼠结肠癌及其肝转移的抑制作用
- Author:
Xiujun LIU
;
Yi LI
;
Yongsu ZHEN
- Publication Type:Journal Article
- From:
Acta Pharmaceutica Sinica
2001;36(1):14-18
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the therapeutic effect of boanmycin (BAM, bleomycin A6) on colon carcinoma 26 and its hepatic metastasis in mice. METHODS A series of models including subcutaneous transplantation, orthotopic transplantation in cecum subserosa, intra-hepatic transplantation of tumor, and intra-splenic transplantation of tumor accompanied with hepatic metastases were employed. In addition, LEICA Q 500IW image analysis system was used to determine the area of metastatic lesions in the liver in histopathological sections. RESULTS BAM at 5 mg*kg-1 and 2.5 mg*kg-1 inhibited the growth of subcutaneous tumors by 78.7% and 61.9%, and the orthotopic tumors by 99.4% and 90.0%; and the intra-hepatic tumors by 86.9% and 75.7%, respectively. Determined by the numbers of metastatic nodules, BAM at 10 mg*kg-1 and 5 mg*kg-1 inhibited hepatic metastases from intra-splenic transplantation by 97.6% and 56.8%; and evaluated by image analysis of metastatic lesions, by 100% and 63.3%, respectively. CONCLUSION Boanmycin is highly effective in a panel of models including the subcutaneous, orthotopic, intra-hepatic transplanted tumors, and hepatic metastases of murine colon carcinoma 26.
