Characteristics of monoclonal antibody against glycophorin A of human erythrocyte
- VernacularTitle:人红细胞血型糖蛋白A单克隆抗体的初步鉴定
- Author:
Xiangdong WU
;
Baotong LI
;
Haifeng SHAO
;
Yonglan ZONG
;
Ming QI
;
Jianguo WU
- Publication Type:Journal Article
- From:
Journal of Medical Postgraduates
2001;14(1):40-41
- CountryChina
- Language:Chinese
-
Abstract:
Objectives: To study and prepare monoclonal antibody against glycophorin A of human erythrocyte(GPA McAb).This antibody is a key reagent in preparation of bispecific antibodies for rapid whole-blood immunoassay. Methods: BALB/c mice received GPA antigen injection. Hybridoma was produced by traditional techniques. Hybridoma was determined with ELISA and indirect agglutination(IA) method. Results: All 3 GPA McAb reacted with GPA, and they did not autoagglutenate with four types of red cells(type A,B,O,AB).Of the 3 McAb-GPA, two antibodies were IgG1,one IgG2 subtypes. Two IgG1 McAb-GPA can be used in making bispecific antibody in preparation of rapid whole-blood immunoassay. Conclusions: Immunogenity of GPA was enhanced after coupling with the native serum albumin of the immunized animal, and high titer GPA McAb could be easily obtained. This result is important in making bispecific antibodies in preparation of rapid whole-blood immunoassay.
