The safety of antigenicity gene transfection with surgical suture into rabbit's tongue muscle
- VernacularTitle:兔舌肌基因转染及其安全性分析
- Author:
Dan HUANG
;
Jinrong LI
- Publication Type:Journal Article
- From:
Journal of Practical Stomatology
2001;17(1):6-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the safety of gene transfection with surgical suture into rabbit's tongue muscle .Methods:pBLacZ gene was transfected into tongue muscle of rabbit by implantation of 1 cm surgical suture,which had been soaked in pBlacZ plasmid solution for overnight.The growth of the muscle cells was analyzed by flow cytometry.The adverse production of anti-double strand DNA was detected by indirect immunofluorescent assay.Result:No difference was found in the growth between gene transfected muscle cells and the controls.No anti-double strand DNA antibody was detected .Conclusion:This gene transfer system may not induce growth change and anti-double strand DNA antibody in the transfected cells.
