THE METHODS FOR QUANTIFYING CAPACITY OF BACTERIA IN DISSOLVING P COMPOUNDS
- VernacularTitle:细菌解磷能力测定方法的研究
- Author:
Xiaorong ZHAO
;
Qimei LIN
;
Yanxin SUN
;
Jun YAO
;
Youshan ZHANG
- Publication Type:Journal Article
- From:
Microbiology
2001;(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Three bacteria of decomposing lecithin and 4 bacteria of dissolving aptite were incubated for 4 weeks with sand media respectively. Phosphorus in the sand was extracted with distilled water and measured by different methods. It was found that the bacteria have a quite different ability to release P from the materials. Part of the P released became organic phosphorus compounds in microbial tissue. However, a large amount of the P was reserved in microbial cells in a form of phosphates. The direct measurement of P in the extract by molybdenum blue method would underestimate the capacity of the bacteria to release P from the materials. The correct approach was that the sand was fumigated with chloroform and then digested with acid before the measurement by molybdenum blue method.
