EFFECT OF FERMENTATION CONDITIONS ON ANTIBIOTIC PRODUCTION OF XENORHABDUS NEMATOPHILUS
- VernacularTitle:嗜线虫致病杆菌产生抗生素的培养基及条件
- Author:
Xiufen YANG
;
Huaiwen YANG
;
Heng JIAN
;
Zheng LIU
- Publication Type:Journal Article
- From:
Microbiology
2001;(1):12-16
- CountryChina
- Language:Chinese
-
Abstract:
The optimum medium and fermentation conditions of the Xenorhabdus nematophilus from Steinernema carpocapsae BJ strain were studied. The relationship between antibiotic activity and pH, reducing sugar, total sugar, amino-nitrogen in process of fermentation was analyzed. The optimal medium contained tryptonl. 5 %, corn powderl o4, soybean flour 3 %, sucrose1 %KH2PO4 0.02 % ,MgSO4 0. 2% and activator 0. 1%, Stock cultured for 16h, inoculum size at 4%(V/V)and primary pH of medium ranged from 6.0 to 8.0, fermentation for 72h were of benefit to the yield of antibiotic. The pH, reducing sugar, total sugar and amino nitrogen in process of fermentation were related to the antibiotic activity. The yield of antibiotic increased by 56. 3 % comparison with nutrient broth.
