Study on targeting therapy of idiopathic thrombocytopenic purpura with MTX-labelled antibody in vitro
- VernacularTitle:氨甲喋呤标记抗体导向治疗自身免疫性血小板减少性紫癜的体外实验研究
- Author:
Zhenyu JIANG
;
Zhiqing ZHANG
;
Xiaofang WANG
;
Weijin CHEN
;
Rongguo NIE
;
Cheng YAO
- Publication Type:Journal Article
- Keywords:
MTX;
IVIG;
Fc receptor;
conjugate
- From:
Journal of Jilin University(Medicine Edition)
2000;26(6):579-581
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To conjugate IVIG and MTX to produce a specific cytotoxicity upon phagocytes.Methods :MTX was conjugated with IVIG by indirect conjugating methods. HSA was used as an intermedi-ary to conjugate MTX with IVIG. The indirect immunofluorescence was adopted to test the binding abilityof Fc fragment. MTT assay was used to measure the cytotoxicity of conjugation on phagocytes. Results:Conjugation showed stronger cytotoxicity upon target cells than free MTX,and it showed only less cyto-toxic effect on Fc receptor negative cells compared with the positive ones. The specific cytotoxicity of IVIG-HSA-MTX was significantly stronger than that of MTX. Conclusion: In vitro the conjugation showed ahighly specific cytotoxicity upon phagocytes.
