Transfection and expressiOn of green fluorescent protein gene in prostate carcinoma PC-3M cell line
- VernacularTitle:EGFP基因在前列腺癌PC-3M细胞系中的转染与表达
- Author:
Hong DENG
;
Xihua CHANG
;
Qian DAI
;
Na WANG
;
Jianju LIU
;
Hong CAO
- Publication Type:Journal Article
- Keywords:
green fluorescent protein;
reporter gene;
lipofectin regant;
prostate
- From:
Journal of Jilin University(Medicine Edition)
2000;26(6):592-594
- CountryChina
- Language:Chinese
-
Abstract:
Objective：To reseach the expression of green fluorescent protein(GFP)gene in prostate car-cinoma PC-3M cell line and the efffect of cell cycle．Methods：GFP gene recombination DNA was construct-ed and PC-3M cells were transfected with lipofectin regant．The expression of GFP was observed under in-vert fluorescence microscope．Meanwhile，the cell cycle of positive cell colon was assayed by flOw cytome-ter．Resuits：After transfection，about 10％～15％cells expressed green fluorescent protein and there wasno significant change compared the cell cycle of positive cell colon with parents cells．Conclusion：GFP sup-plys a new way in reseaching metabolic phenomenon of live cells．
