THE EFFECTS OF A CONTROLLED pH ENVIRONMENT ONPOLYSACCHARIDE SYNTHESIS BY AUREOBASIDIUM PULLULANS
- VernacularTitle:控制pH环境对出芽短梗霉胞外多糖合成的影响
- Author:
Hanbo ZHANG
;
Lizhong CHENG
;
Tao SHA
;
Huasun DING
;
Zhiwei ZHAO
- Publication Type:Journal Article
- From:
Microbiology
2001;(1):35-38
- CountryChina
- Language:Chinese
-
Abstract:
The effect of pH on the extracellularpolysaccharide synthesis by Aureobasidium pullulans was studied by addding CaCO3and HCI. Cultivated in P2 liquid medium for 24h,pH dropped to 3. 6 because of strongly producing acid. Under this low pH environment, further fermentation for 120h,only 5. 9g/L of polysacharide was obtained. When grown in MP2 medium contaning0. 5 % CaCOs,the pH was kept above 5.0 during 144 hours,production of polysaccharide increased to 31g/L. The detailed information of effects of controlled pH on polysaccharide production showed an optimal pH value 5. 0 must be maintained through the fermentative period.
