MODIFIED TECHNIQUES FOR PURIFICATION OF ALFALFA MOSAIC VIRUS
- VernacularTitle:苜蓿花叶病毒提纯方法的改进
- Author:
Jishuang CHEN
;
Mingguang FENG
- Publication Type:Journal Article
- From:
Microbiology
2001;(1):68-72
- CountryChina
- Language:Chinese
-
Abstract:
Three purification methods ,based on differential centrifugation,precipitation by polyethylene glycol (PEG)and ultra-speed centrifugation,were compared for purification of an alfalfa mosaic virus (AMVSY)previously isolated from Trifolium repens. The Purified virus was observed under electron microscope, measured,by ultra-violet absorpton analysis and protein determination with SDS-polyacrylamide gel electrophoresis. Our results showed that a method by alternative use of sodium phosphate buffers containing 0. 1mol/L EDTA and 0. 1mol/L MgSO4 achieved the best purification with less miscellaneous protein contamination,integrate virus particles and relatively high yield, which was of 47.6mg virion per hundred grams of fresh leaves of Chanopodium quinoa inoculated with AMV-SY. The coat protein of AMY-SY was tested for about 29 kilo-Dalton.
